Omics · study · 2026
Comparative transcriptomics reveals differences in cortical cell type organization between metatherian and eutherian mammals [snRNA-Seq]
Listed in NCBI GEO
The neocortex supports mammalian cognition through a conserved columnar architecture, but variation in the transcriptomic organization of cortical columns across deep mammalian divergences has not been directly examined.
Description
We used single-nucleus RNA sequencing and spatial transcriptomics to compare primary visual cortex in marsupial (opossum) and placental (mouse) mammals. Major neuronal subclasses and laminar architecture were broadly conserved, but intratelencephalic (IT) neurons differed markedly.
Opossum IT neurons exhibited more generalized, overlapping transcriptomic identities with a continuous spatio-transcriptomic gradient between superficial (L2/3) and deep (L5) IT populations, whereas mouse IT neurons showed sharper transcriptomic distinctions and greater spatial confinement, consistent with increased specialization. Mouse cortex also displayed reduced PV interneuron density and redistribution of perineuronal nets, suggesting altered constraints on cortical plasticity.
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These findings demonstrate that substantial variation in intracortical circuit organization exists across deeply diverged mammalian lineages, identifying IT neurons as a principal axis of evolutionary divergence in the neocortical column.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE299nnn/GSE299387 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE299387 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1274239 ncbi.nlm.nih.gov/bioproject/PRJNA1274239 ↗
project · from NCBI GEO
- PubMed 42005962 pubmed.ncbi.nlm.nih.gov/42005962 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE299387 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |