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Omics · study · 2026

Comparison of global mRNA decay at single-gene resolution in E. coli Δrnb Δrnr versus E. coli Δrnb Δrnr Δfbp

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We determined whether deletion of the glycolysis gene fbp broadly modulates transcript stability in a strain where PNPase is the sole exoribonuclease.

Description

We analyzed global RNA degradation at single-gene resolution after rifampicin treatment of E. coli Δrnb Δrnr Δfbp versus the isogenic parental strain, E. coli Δrnb Δrnr, using RNA sequencing. ERCC spike-in standards were incorporated before cDNA library preparation and used for normalization of read-counts-per-gene.

We found 1321 transcripts with significantly different degradation patterns between the strains.

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Expression profiling by high throughput sequencing
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Life Sciences
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RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 8 field assertions
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NCBI GEOGSE31507015 d agoJSON v1
FieldAssertionExtractorEvidence
access_levelsource · NCBI GEOconnector:ncbi_geo@1.0.0
concepts[field].local:field:life-sciencesmapping · NCBI GEOconnector:ncbi_geo@1.0.0
concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencingsource · NCBI GEOconnector:ncbi_geo@1.0.0/gdstype
concepts[modality].local:modality:rna-seqenrichment · NCBI GEOkeyword-concept-rules@1.0.0title+description (75%)
concepts[modality].local:modality:sequencingenrichment · NCBI GEOkeyword-concept-rules@1.0.0title+description (75%)
descriptionsource · NCBI GEOconnector:ncbi_geo@1.0.0/summary
publication_datesource · NCBI GEOconnector:ncbi_geo@1.0.0
titlesource · NCBI GEOconnector:ncbi_geo@1.0.0/title