Integrated imaging and molecular biology dataset for the characterization of a putative anti-eukaryotic effector from the Xanthomonas citri type VI secretion system

The data were obtained using a combination of molecular biology, biochemical, NGS, and imaging-based approaches, including blotting techniques, and microscopy. Quantitative data include measurements such as area, time, signal intensity, and relative abundance of biological molecules, while qualitative data consist of microscopy images and experimental videos.

Experiments were conducted using the bacterial model X. citri subsp. citri and the eukaryotic model organisms Dictyostelium discoideum and Saccharomyces cerevisiae, enabling the assessment of effector activity across different biological systems. Whenever applicable, experiments were performed in biological triplicates to ensure statistical robustness. Data acquisition was carried out using multi-user equipment from the Institute of Biology at the University of Campinas (IB-UNICAMP), with additional use of facilities like National Institute of Photonics Applied to Cell Biology (INFABiC), Laboratório Central de Tecnologias de Alto Desempenho em Ciências da Vida (LaCTAD), and NGS soluções genômicas.