Transcriptome profiling of peripheral blood mononuclear cells from patients with alcohol-related hepatitis following antioxidant and trace element supplementation

PBMCs from 10 patients were cultured for 48 hours with no supplementation or supplemented with N-acetylcysteine (NAC), selenium or zinc sulfate. Genome-wide gene expression was assessed using the Affymetrix Clariom S Human HT microarray.

Each patient sample was analysed under all four culture conditions, allowing paired comparison of supplemented and unsupplemented PBMCs. Expression data were processed using robust multi-array average (RMA) normalisation in R/Bioconductor and differential expression was assessed using limma with patient identity included as a blocking factor.