Single-nucleus transcriptomic profiling of adult male Japanese medaka brain following parental benzo[a]pyrene exposure

This study examined whether parental BaP exposure produces transcriptional changes in the brains of unexposed adult first filial generation (F1) Japanese medaka. Adult parental fish were exposed to solvent control or 1 µg/L BaP for 21 days; eggs were collected after exposure and F1 offspring were reared in clean water.

Whole brains from three control-lineage and three BaP-lineage adult males were analyzed by single-nucleus RNA sequencing. Fourteen transcriptionally distinct brain cell populations were resolved, and treatment-associated differential expression was evaluated within each cluster.