Association between Alcohol Consumption and Parkinson’s Disease Risk and Identification of RIT2 as a Linked Biomarker

On the experimental day, body weight was recorded. Approximately 3 hours after the onset of the dark phase, the original drinking bottle was removed from each cage, and the mice were provided with either an ethanol solution or a diluted baijiu solution for ad libitum access for 4 hours. The mice were randomly divided into three groups (n = 4 per group): the control group (tap water), the 20% (v/v) pure ethanol group, and the 20% (v/v) diluted baijiu group.Immediately after the drinking session, brain tissues were collected.

Quantitative real-time PCR (qPCR) was performed using the QuantStudio 1 Plus Real-Time PCR System (Thermo Fisher Scientific, USA) with SYBR Green reagent. The relative expression levels of target genes were analyzed using the 2⁻ΔΔCt method. Following amplification, melting curve analysis was conducted to confirm the specificity of the amplified products.

The target gene examined was RIT2, with β-actin used as the internal reference gene.DATA3_RNAseq_gene_tpmImmediately after the drinking session, mouse brain tissues were collected. RNA sequencing was performed using the Illumina NovaSeq X Plus platform to obtain TPM expression data. Three groups (n = 4 per group): the control group (tap water), the 20% (v/v) pure ethanol group, and the 20% (v/v) diluted baijiu group.DATA4_DEG_ControlvsAlcoholDifferentially expressed genes：the control group (tap water) vs. the 20% (v/v) pure ethanol group.DATA5_DEG_ControlvsBaijiuDifferentially expressed genes：the control group (tap water) vs. the 20% (v/v) diluted baijiu group.