The raw data for the manuscript "Evolutionary and multi-omic divergences associated with the extreme sexual size dimorphism in spiders"

To minimize potential confounding effects associated with developmental stage, sampling location, and collection time, we included only mature individuals collected under these standardized conditions. For transcriptomic profiling by RNA sequencing (RNA-seq), we dissected seven tissues from female and male spiders, including brain, abdominal skin, carapace skin, sternum skin, leg, palp, and venom gland.

We generated three biological replicates for each sex–tissue combination, with each replicate consisting of pooled tissue samples from three adult individuals of the same sex. Immediately after dissection, we transferred all tissue samples into RNase-free 1.5 mL microcentrifuge tubes, flash-froze them in liquid nitrogen, and stored at −80 °C until RNA extraction and sequencing. For assay for transposase-accessible chromatin using sequencing (ATAC-seq), we generated chromatin accessibility profiles from whole body samples of four adult individuals, including two females and two males.