Data Sheet 1_A study on oral microbiome characteristics and differences in epilepsy adults with depressive symptoms based on 16S rRNA sequencing.xlsx

Using an uncorrected p value < 0.05 as the screening criterion, microbial composition analysis revealed that Dialister and Olsenella were enriched in EDS, while Bacillus, Fusicatibacter, and [Eubacterium]_coprostanoligenes_group were enriched in EP. LEfSe analysis further suggested that Dialister and Bacillus were key differential genera with discriminatory capability. Based on PICRUSt2’s functional prediction results, the predicted functional abundance annotated to the RIG-I-like receptor signaling pathway was higher in the EDS group than in the EP group.

Multivariate Firth-corrected logistic regression analysis showed that the abundance of Dialister remained associated with EDS (OR = 1.646, 95% CI: 1.007–2.896, p = 0.047), after adjusting for potential confounding factors.</p>Conclusion<p>This study utilizing 16S rRNA sequencing elucidates alterations in the oral microbiome associated with epilepsy and concurrent depressive symptoms. Significantly, the enrichment of Dialister is independently correlated with depressive symptoms following adjustment for confounding variables, indicating its potential as a microbial biomarker.

Functional prediction analysis further indicated that the associated oral microbial alterations may involve innate immune-related functional characteristics, particularly the RIG-I-like receptor signaling pathway. These findings provide new clues for further investigating the potential association between oral microbiota and epilepsy with depressive symptoms.</p>