Excel · study · 2026
Oxidative stress alters miRNA content in stressed granulosa cells and their derived extracellular vesicles
Listed in NCBI GEO
Granulosa cells (GCs) play a central role in oocyte maturation and follicular development, and their function is very sensitive to oxidative stress, which can compromise female fertility.
Description
Extracellular vesicles (EVs) released by GCs serve as intercellular messengers and are known to carry bioactive molecules, including microRNAs (miRNAs), which can modulate stress responses and cellular communication within the follicular environment.
This study aimed to characterize and compare the miRNA profiles of both GCs and their secreted EVs following exposure to oxidative stress. Bovine GCs were treated with 5 µM H₂O₂ for 40 min, followed by 24 hours of culture. EVs were isolated from the conditioned media using size-exclusion chromatography and characterized by nanoparticle tracking analysis, transmission electron microscopy, and flow cytometry.
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Small RNA sequencing was performed on both the cells and their secreted EVs. Exposure to oxidative stress increased intracellular ROS levels and decreased mitochondrial activity in GCs. Differential expression analysis revealed no significant changes in miRNA abundance in GC in response to oxidative stress.
In GC derived EVs, however, 10 miRNAs were significantly higher abundant, including miR-134, miR-10175-5p, miR-197, miR-2284h-5p, miR-2284y, miR-2285av, miR-2285au, miR-369-5p, miR2411-5p and miR-2387. Functional enrichment analysis of predicted targets for EV-miRNAs revealed involvement in processes such as angiogenesis, cell migration as well as WNT, MAPK and Oxitocin signaling. These findings suggest that oxidative stress alters the miRNA cargo of EVs, potentially affecting intra-follicular communication and stress adaptation.
This dual characterization provides new insights into the regulatory role of EV-derived miRNAs in ovarian physiology under oxidative conditions.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE304nnn/GSE304292 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE304292 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1300294 ncbi.nlm.nih.gov/bioproject/PRJNA1300294 ↗
project · from NCBI GEO
- PubMed 41882814 pubmed.ncbi.nlm.nih.gov/41882814 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Non-coding RNA profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Microscopy 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE304292 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:non-coding-rna-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:microscopy | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |