Omics · study · 2026
Transcriptomic profiling of mouse subgranular zone-derived neural stem cells exposed to cadmium
Listed in NCBI GEO
We performed RNA-seq to investigate the effects of cadmium (Cd) exposure on the transcriptome of primary mouse neural stem cells (NSCs) isolated from the subgranular zone (SGZ) of the hippocampus.
Description
NSCs at passage 3 were exposed to 0.8 μM CdCl₂ for 72 hours, and total RNA was extracted for sequencing on an Illumina platform. Differential expression analysis identified 684 differentially expressed genes (334 downregulated, 350 upregulated).
Gene Ontology enrichment analysis of downregulated genes revealed impairment of neurogenesis-related processes, and WikiPathways analysis indicated enrichment of Wnt signaling pathway components. These transcriptomic data support the finding that Cd disrupts non-canonical Wnt5a signaling and impairs neuronal differentiation of SGZ-derived NSCs.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328645 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328645 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1456061 ncbi.nlm.nih.gov/bioproject/PRJNA1456061 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE328645 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |