Data · dataset · 2026
Microbial community assembly in rapeseed under uranium stress: 16S rRNA sequencing across soil-root-leaf niches
Listed in ScienceDB
This dataset comprises 16S rRNA gene amplicon sequencing data generated from a pot experiment investigating microbial community dynamics under uranium stress in rapeseed (Brassica napus).
Description
Soil samples were collected from three treatments: original soil, sterilized soil, and sterilized-inoculated soil, with or without 150 mg kg⁻¹ uranium addition. Four ecological niches were sampled: bulk soil, rhizosphere soil, root endosphere, and leaf phyllosphere.
The V3–V4 region of the 16S rRNA gene was amplified using primers 338F/806R and sequenced on the Illumina MiSeq platform (2×300 bp paired-end). Raw reads were quality-filtered, denoised, and merged using the DADA2 pipeline, and operational taxonomic units (OTUs) were clustered at 97% similarity. Taxonomic assignment was performed against the Greengenes 13.8 database.
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Quality control included negative controls (extraction blanks and PCR negatives) and rarefaction to 95% of the minimum sample depth to ensure comparability. These data are valuable for studying microbial community assembly, niche differentiation, functional redundancy/compensation, and plant–microbe interactions in uranium-contaminated soils, and can support comparative analyses with other heavy-metal or phytoremediation studies.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.43243 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Evolutionary biology 73% · Sequencing 75%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.43243 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:3104 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (73%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |