Establishment and characterization of a mouse model with chronic pneumonia infected by <i>Pseudomonas aeruginosa</i>

However, constructing an animal model for chronic infection remains challenging, hindering research advancement in this field.</p> <p>This study employed microfluidic technology to prepare alginate-encapsulated P. aeruginosa microspheres and established a chronic pneumonia model in C57BL/6J mice following aerosolized intratracheal (i.t.) delivery of the encapsulated bacteria.

Comprehensive evaluation included bacterial load measurement, histopathological analysis by hematoxylin and eosin (H&E) staining, inflammatory cytokine detection by enzyme-linked immunosorbent assay (ELISA), immune cell profiling (flow cytometry), and transcriptomic analysis by RNA sequencing (RNA-seq).</p> <p>We successfully established a mouse model of P. aeruginosa-induced chronic pneumonia with persistent bacterial colonization (~2,000 colony-forming units (CFU)/g at day 14), consistent histopathological features across independent experiments, and sustained elevation of inflammatory cytokines.