Single-cell transcriptomic analysis of hepatic immune cells from untreated and ConA-treated mice

In this study, we established a low-dose concanavalin A (ConA)-induced immune tolerance model in mice and performed single-cell RNA sequencing (scRNA-seq) on hepatic immune cells isolated from untreated (UT) and ConA-treated groups. Transcriptomic profiling revealed substantial remodeling of the hepatic immune microenvironment following low-dose ConA stimulation.

Notably, we observed a pronounced activation of type II immune responses, characterized by enrichment of Th2-associated cytokine signatures and expansion of Th2, Tfh subsets. Further analysis identified B cells as potential key contributors to this process, exhibiting distinct transcriptional programs associated with humoral immunity that may play a role in the establishment of immune tolerance.