Fecal metabolomics,16S rRNA sequencing, metagenomics, metaproteomics and single-cell sequencing

Subsequently, fecal microbiota from 20 randomly selected human donors (10 per group) was transplanted into 20 germ-free mice, followed by single-cell sequencing analysis of colonic tissues. The fecal samples from these 10 acromegaly patients and 10 control subjects were then analyzed using metagenomics and metaproteomics.

Additionally, intestinal polyp samples from 6 acromegaly patients and 6 control patients were selected for proteomic analysis. Finally, target bacteria were transplanted into germ-free mice, and single-cell sequencing analysis was performed on the colonic tissues of these mice.