Excel · study · 2026
Transcriptomic effects of miR-21-5p on preimplantation mouse blastocysts
Listed in NCBI GEO
Preimplantation embryos cultured in vitro exhibit increased stress and apoptosis compared with in vivo development. miR-21-5p is a conserved, highly abundant microRNA in reproductive-tract extracellular vesicles and has been implicated in cell-survival pathways.
Description
To define its transcriptomic impact on early embryos, we performed RNA-seq on mouse blastocysts generated by IVF and cultured in KSOM with either miR-21-5p agomir (50 nM) or blank control.
Each biological replicate consisted of five pooled blastocysts; n=4 replicates per group. Sequencing was carried out on an Illumina NovaSeq X Plus.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308541 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308541 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1330846 ncbi.nlm.nih.gov/bioproject/PRJNA1330846 ↗
project · from NCBI GEO
- PubMed 42185219 pubmed.ncbi.nlm.nih.gov/42185219 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE308541 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |