Data · dataset · 2024
Softness limits CTLs self-killing and is inherited to T-leukemic cells for immune escape
Listed in ScienceDB
Using CRISPR/Cas9-based gene overexpression method, we screened membrane or membrane-related protein molecule(s) by transfecting Jurkat cells with membrane protein gRNA library, followed by perforin treatment and PI staining.
Description
The top 10% among PI staining high and the bottom 10% among PI staining negative cells were sorted for next-generation sequencing (NGS) . Using the MAGeCK algorithm or RRA analysis, top sgRNAs were identified in PIh cells . We provide evidence that effector CTLs exhibit the perforin-resistant softness by downregulating cytoskeletal protein filamin A (FLNA)and this FLNA-dependent pathway is usurped by leukemic T cells to evade T cell killing.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.14308 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.14308 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |