Omics · study · 2026
RNA-seq of WT and Rbm25-deficient peritoneal macrophages infected with vesicular stomatitis virus (VSV)
Listed in NCBI GEO
The purpose of this study is to detect differentially expressed genes in wild type (WT) and Rbm25-deficient peritoneal macrophages (PMs) infected with vesicular stomatitis virus for 4 hours.
Description
Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Mouse PMs from WT and Rbm25-deficient mice were cultured in DMEM medium.
PMs were stained to confirm the surface expression of CD11b and F4/80. Cells with purity >97.5% were used for subsequent experiments. WT and Rbm25-deficient PMs were infected with vesicular stomatitis virus for 4 hours, of which RNA profiles were generated by deep sequencing, using Illumina.
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We identified several differently expressed genes in the two groups of cells.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314891 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE314891 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1393090 ncbi.nlm.nih.gov/bioproject/PRJNA1393090 ↗
project · from NCBI GEO
- PubMed 42299815 pubmed.ncbi.nlm.nih.gov/42299815 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE314891 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |