Data · dataset · 2025
Replication Data for: Micro-tube reusability protocol for DNA shearing: an economical and eco-friendly option for DNA library preparations
Listed in Borealis
The following dataset(s) are part of an experimental study testing the efficiency and reusability of micro-tubes specifically designed to be used on a Covaris - M220 sonication device.
Description
This device shears genomic DNA into size-specific fragments, which is a critical step in the preparation of genomic libraries for high-throughput sequencing. Here, we archive two datasets, one on the concentrations of DNA left in tubes after each step of a four-step cleaning protocol, and another listing the shearing peak (in bp) produced in 10 reused tubes that were each used five times.
The data are used in the scripts MicroTube.R and Shearingrep.R, available on GitHub.
Links
Where it is published
- Dataverse dataset page borealisdata.ca/dataset.xhtml?persistentId=doi%3A10.5683%2FSP3%2FUGBOIX ↗
landing page · from borealisdata ca
- DOI doi.org/10.5683/sp3/ugboix ↗
DOI / persistent id · from borealisdata ca
Catalogue records · 1
- Dataverse API borealisdata.ca/api/datasets/:persistentId/?persistentId=doi%3A10.5683%2FSP3%2… ↗
metadata API · from borealisdata ca
Topics
- Stated by source
- Agricultural Sciences · Earth and Environmental Sciences · Medicine, Health and Life Sciences · Other
- From keywords
- Earth & Environmental Science · Life Sciences · Medicine & Health
- Inferred from text
- Medical biotechnology 69% · Sequencing 75%
Provenance · 1 source records, 15 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Borealis | doi:10.5683/SP3/UGBOIX | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].anzsrc:group:3206 | enrichment · borealisdata ca | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].dataverse_subject:agricultural-sciences | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].dataverse_subject:earth-and-environmental-sciences | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].dataverse_subject:medicine-health-and-life-sciences | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].dataverse_subject:other | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].local:field:earth-environmental | mapping · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].local:field:life-sciences | mapping · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].local:field:medicine-health | mapping · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[modality].local:modality:sequencing | enrichment · borealisdata ca | keyword-concept-rules@1.0.0 | title+description (75%) |
| created_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| description | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /description |
| publication_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| title | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /name |
| updated_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| version_label | source · borealisdata ca | connector:borealisdata_ca@1.0.0 |