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[Acropora cervicornis transcriptomes: nutrient- and disease-exposed] - Acropora cervicornis transcriptomes: nutrient- and disease-exposed from samples collected at Mote Marine Laboratory in situ nursery from June to July 2022 (Collaborative Research: Tracking the interacting roles of the environment, host genotype, and a novel Rickettsiales in coral disease susceptibility)

Listed in Biological and Chemical Oceanography Data Management Office (BCO-DMO) ERDDAP

We confirmed data from previous studies showing that genotypes of Acropora cervicornis dominated by the coral parasite Aquarickettsia are more susceptible to White Band Disease than genotypes with diverse microbiomes.

Description

However, in a study with ramets from genotypes exhibiting each microbiome profile, we found that the strongest predictor of disease development was prior exposure to increased thermal stress that occurred independent of the study on Florida's reef during the experiment.

While no ramets of the coral genotype with a diverse microbiome developed disease after exposure to nutrient enrichment, seven ramets of this genotype developed disease after exposure to elevated temperatures and subsequent disease challenge. Two ramets exposed to elevated temperatures and not exposed to disease also died, suggesting that temperature alone could have been fatal to this genotype. An equal number of ramets exposed to elevated nutrients and elevated temperatures developed disease in the genotype dominated by Aquarickettsia.

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To evaluate the effect of Aquarickettsia infection; nutrient enrichment; disease exposure; and the combination of these factors on coral immune function, we conducted RNAseq on 42 samples to an averaging sequencing depth of 22,168,890.6 reads per sample (paired-end).

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Inferred from text
Disease 75% · RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 7 field assertions
SourceKeyLast seenRaw
Biological and Chemical Oceanography Data Management Office (BCO-DMO) ERDDAPbcodmo_dataset_924760_v112 d agoJSON v1
FieldAssertionExtractorEvidence
access_levelsource · erddap bco dmo orgconnector:erddap_bco_dmo_org@1.0.0
concepts[disease].local:disease:diseaseenrichment · erddap bco dmo orgkeyword-concept-rules@1.0.0title+description (75%)
concepts[field].local:field:ocean-atmosphericmapping · erddap bco dmo orgconnector:erddap_bco_dmo_org@1.0.0
concepts[modality].local:modality:rna-seqenrichment · erddap bco dmo orgkeyword-concept-rules@1.0.0title+description (65%)
concepts[modality].local:modality:sequencingenrichment · erddap bco dmo orgkeyword-concept-rules@1.0.0title+description (75%)
descriptionsource · erddap bco dmo orgconnector:erddap_bco_dmo_org@1.0.0/summary
titlesource · erddap bco dmo orgconnector:erddap_bco_dmo_org@1.0.0/title