Excel · study · 2026
RUNX2 Promotes Fibrotic Activation in Skin Fibroblasts and Is Targetable by Small-Molecule Inhibition
Listed in NCBI GEO
Fibrosis is driven by activated fibroblasts, but key transcriptional regulators remain unclear.
Description
We investigated the role of RUNX2 using three human skin fibroblast lines with RUNX2 knockdown (shRUNX2) and matched controls (shControl), treated with or without TGF-β, followed by bulk RNA sequencing. RUNX2 depletion attenuated TGF-β–induced pro-fibrotic gene programs, including extracellular matrix organization and myofibroblast differentiation.
In primary fibroblasts, the small molecule F0565-0303 reduced TGF-β–induced fibrotic signatures and decreased RUNX2 expression, partially mimicking RUNX2 knockdown, while showing minimal effects under basal conditions. These results identify RUNX2 as a key driver of fibroblast activation and support its pharmacological targeting as an anti-fibrotic strategy.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330910 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330910 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1465908 ncbi.nlm.nih.gov/bioproject/PRJNA1465908 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE330910 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |