Omics · study · 2026
Targeting phosphodiesterase 8A (PDE8A) and adipogenesis to promote hair regeneration
Listed in NCBI GEO
To maximally capture adipocytes and hair follicle keratinocytes, which are largely lost through enzymatic digestion in scRNA-seq, we performed single-nuclei RNA sequencing (Sn-RNAseq) of the developing mouse skin samples.
Description
The aim of this analysis was to identify key cell-cell interactions between adipocytes and hair follicle keratinocytes. Briefly, dorsal skin biopsies were collected from the developing C57BL/6 pups (at postnatal day 2) and 10x Genomics Sn-RNAseq was applied to identify distinct cell populations.
After low quality nuclei and outliers were discarded, ～16481 nuclei were used for downstream bioinformatic analyses, including Unsupervised clustering, marker gene expression, and Cell-Chat signaling network analyses. Overall, our results reveal that adipocytes secrete growth factor to support hair follicle growth.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE272nnn/GSE272917 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE272917 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1135731 ncbi.nlm.nih.gov/bioproject/PRJNA1135731 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE272917 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |