Excel · study · 2026
RNA-seq analysis of Nogo-B knockdown in human dermal lymphatic endothelial cells under TGF-β2 stimulation
Listed in NCBI GEO
Nogo-B (RTN4B), an endoplasmic reticulum-associated protein, plays a critical role in regulating endothelial cell function and vascular remodeling.
Description
However, its role in lymphatic endothelial cells (LECs) and in response to profibrotic signaling remains poorly understood. Transforming growth factor-β2 (TGF-β2) is a key regulator of endothelial phenotypes and has been implicated in lymphatic dysfunction under pathological conditions.
In this study, we performed RNA sequencing (RNA-seq) to investigate the transcriptional changes associated with Nogo-B knockdown in human dermal lymphatic endothelial cells (HDLECs) under TGF-β2 stimulation. HDLECs were transduced with shRNA targeting Nogo-B (shNogo-B) or control shRNA (shGFP), followed by treatment with TGF-β2 for 72 hours. Total RNA was extracted and subjected to high-throughput sequencing.
Read the rest (2 more)
Comparative transcriptomic analysis was conducted to identify differentially expressed genes and pathways regulated by Nogo-B under TGF-β2-stimulated conditions. This dataset provides insights into the molecular mechanisms by which Nogo-B modulates lymphatic endothelial cell responses to TGF-β signaling, including pathways related to extracellular matrix organization, cell migration, proliferation, and cytoskeletal remodeling.
These data serve as a resource for understanding the role of Nogo-B in lymphatic endothelial biology and its contribution to TGF-β-mediated signaling and dysfunction.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330702 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330702 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1465291 ncbi.nlm.nih.gov/bioproject/PRJNA1465291 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE330702 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |