CUT&Tag sequencing datasets of ZmIDD30

Maize protoplasts were isolated from the second leaves of 14‑day‑old etiolated seedlings and transiently transformed with a ZmIDD30‑GFP fusion plasmid, using an empty GFP plasmid as control. Three independent biological replicates were conducted for each group.

For CUT&Tag library preparation, the Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro (TD904, Vazyme) was used. Transformed protoplasts were immobilized on ConA Beads Pro and incubated overnight at 4 °C with rabbit anti‑GFP primary antibody (1:100, Abmart), followed by goat anti‑rabbit IgG secondary antibody (1:100, Vazyme). After treatment with pA/G‑Tn5 transposase and tagmentation, DNA fragments were extracted, PCR‑amplified, purified, and sequenced on an Illumina NovaSeq 6000 platform.