MELK inhibition promotes megakaryopoiesis and platelet recovery in immune thrombocytopenia through ERK–cofilin signaling

To profile MELK-dependent gene expression changes, we performed bulk RNA-seq on the human megakaryoblastic cell line MEG-01 transduced with a control pLKO.1 vector or a MELK-targeting shRNA (sh2-MELK), with three independent biological replicates per condition.

Paired-end sequencing reads (raw FASTQ files) and processed gene-level expression quantifications are provided to support differential expression and pathway analyses of MELK knockdown in megakaryocytic cells.