Omics · study · 2026
Whole-transcriptome sequencing of NT2.5, NT2.5LM, and NT2.5LV: a HER2-positive murine breast cancer cell line series with lung- and liver-preferential metastasis
Listed in NCBI GEO
The NT2.5 cell line and its lung-preferential (NT2.5LM) and liver-preferential (NT2.5LV) metastatic derivatives constitute an isogenic HER2-positive murine breast cancer model derived from neu-N transgenic FVB/N mice.
Description
To define the transcriptional programs associated with organ-preferential metastatic colonization, we performed bulk poly(A) RNA-sequencing on the three cell lines grown in vitro. Differential expression analysis identified divergent, organ-correlated transcriptional programs distinguishing the lung- and liver-preferential derivatives on a conserved genomic background.
These data accompany the whole-genome sequencing of the same cell line series deposited under the same BioProject.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336999 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336999 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1483685 ncbi.nlm.nih.gov/bioproject/PRJNA1483685 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · Genome sequencing 65% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE336999 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:genome-sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |