Multiomic and integrative analysis of human and murine AML data reveal a stem-like profile of Phf19-depleted cells

Such disease may originate by the expression of oncogenic chimeric proteins, like MLL-AF9. Many works molecularly characterized the final stage of the leukemias, but fewer it’s known about the initiation process and the impact that cell-intrinsic factors (like epigenetics) may have on its development.

Indeed, studies focusing on early leukemogenic stages in epigenetically altered hematopoietic cells are lacking. Recently, we characterized the role of a Polycomb-associated protein, called Phf19 in healthy hematopoietic cells, whose depletion (Phf19KO) compromises the hematopoietic stem cells (HSCs) functioning, like cell state regulation and maintenance. Here, we expressed the MLL-AF9 oncogene in Phf19KO hematopoietic cells and generated multiomic data using bulk and single cell NGS technologies.