Bulk RNA sequencing of THP-1-derived macrophage-like cells with stable PGK1 overexpression versus empty vector control

THP-1 cells stably expressing either an empty vector or PGK1 were generated via lentiviral infection (pLVX constructs; puromycin selection). Cells were stimulated with 150 nM phorbol 12-myristate 13-acetate (PMA) for 24 h to induce differentiation into M0 macrophages, followed by 20 ng/mL IL-4 and IL-13 for 24 h, before RNA extraction.

Total RNA was isolated with the RNAsimple Total RNA Kit (FOREGENE) and all six libraries were sequenced in a single batch at Lianchuan (Hangzhou, China).