Omics · study · 2026
Effect of NF-ĸB and JAK inhibition in a 3D spheroid model of rheumatoid arthritis (RA) synovial tissue incorporating RA fibroblast-like synoviocytes, macrophages and endothelial cells
Listed in NCBI GEO
This study uses a human multicellular 3D spheroid model to investigate inflammatory and angiogenic mechanisms in rheumatoid arthritis (RA) synovial tissue and to evaluate responses to targeted kinase inhibitors.
Description
Human M1- or M2-polarized macrophages were co-cultured with RA fibroblast-like synoviocytes (RAFLS) and endothelial cells (HUVECs) to generate spheroids embedded in a collagen-based matrix (n=4). Spheroids were exposed to inflammatory stimuli (20%SF) and pharmacological inhibitors including JAK1/3 inhibitor tofacitinib (5μM) and NF-ĸB pathway inhibitors IKKβi (2.5μM) and NIKi (5μM).
Bulk-RNA sequencing was performed to characterize transcriptional responses across experimental conditions.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325085 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325085 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1438607 ncbi.nlm.nih.gov/bioproject/PRJNA1438607 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE325085 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |