Omics · study · 2026
Dysregulated Cholesterol Crystal-STING Pathway in Microglia Drives Neuroinflammation after Ischemic Stroke
Listed in NCBI GEO
Dysregulated lipid metabolism is increasingly implicated in microglial activation, yet the specific consequences of massive cholesterol overload following permanent ischemia remain elusive.
Description
Using single-cell RNA sequencing in permanent distal middle cerebral artery occlusion (dMCAO) and perioperative ischemic stroke (PIS) models, we elucidated the pathogenic role of intracellular cholesterol crystals. We identified a progressive accumulation of cholesterol crystals within peri-infarct microglia during the subacute phase, driven by a specific downregulation of the lipid-processing enzyme lipoprotein lipase (LPL).
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313515 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313515 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1379866 ncbi.nlm.nih.gov/bioproject/PRJNA1379866 ↗
project · from NCBI GEO
- PubMed 42578421 pubmed.ncbi.nlm.nih.gov/42578421 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE313515 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |