Single-nucleus RNA sequencing of brain tissue from HIV-infected and uninfected donors.

Postmortem brain tissues from uninfected controls, viremic individuals, and antiretroviral therapy (ART) suppressed people with HIV were dissociated into single-nucleus suspensions and profiled by high-throughput RNA sequencing. The resulting dataset supports the identification of transcriptional reprogramming, immune modulation, and latent viral reservoir signatures associated with HIV-associated neurocognitive disorders (HAND).

Integration of single-nucleus RNA-seq data across these clinical conditions provides a comprehensive atlas of HIV host interactions in the central nervous system.