Data · dataset · 2026
Longitudinal transcriptomic profiles of the small intestine and colon across the lifespan in a neonatal overfeeding mouse model
Listed in ScienceDB
We used an established neonatal overfeeding model by reducing litter size, assigning litters to either 4 pups per dam (Overfed group) or 8 pups per dam (Normal-fed control group).
Description
To longitudinally bridge early-life epithelial programming with later-life aging phenotypes at the transcriptomic level, small intestine and colon tissues were harvested from independent cohorts of mice at 3 weeks, 6 months, and 28 months of age.
Total RNA was isolated from all aforementioned tissues using TRIzol Reagent followed by DNase I treatment (Takara) to remove genomic DNA contamination. RNA integrity and purity were verified using an Agilent 5300 Bioanalyzer (Agilent Technologies), with all samples exhibiting RIN values > 8.0. Library preparation was performed by Shanghai Majorbio Bio-pharm Biotechnology Co., Ltd using the Illumina Stranded mRNA Prep, Ligation protocol, followed by 150 bp paired-end sequencing on an Illumina NovaSeq 6000 platform.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.33052 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Longitudinal study 65% · Sequencing 75% · Zoology 68%
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.33052 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:3109 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (68%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[method].local:method:longitudinal-study | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |