Data · dataset · 2025
Raw data for genome assembly of Myrica nana
Listed in ScienceDB
Fresh young leaves used for genome sequencing were collected from the M. nana plant grown in the wild environment of Guizhou, China.
Description
The total genomic DNA for each of the accessions was extracted from leaves using the CTAB method[20]. A second-generation sequencing library was constructed using the DNBSEQ-T7 platform (MGI, Shenzhen, China), yielding 17.97 Gb of raw data.
High-quality genomic DNA was used to construct a SMRTbell library with insert sizes of 15-20 kb, which was sequenced on the PacBio Sequel II platform, generating 16.15 Gb of long-read data. To assist in genome scaffolding and chromatin interaction analysis, a Hi-C library was prepared and sequenced on the same DNBSEQ-T7 platform, resulting in 25.63 Gb of valid data after quality control. In addition, total RNA was extracted from four tissues (root, stem, leaf, flower, and fruit) using TRIzol reagent (Vazyme, China).
Read the rest (1 more)
A cDNA library with an insert size of 350 bp was constructed using the MGI Easy RNA Library Prep Kit and subjected to paired-end sequencing on the DNBSEQ-T7 platform, producing 37.77 Gb of transcriptomic raw data.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.34523 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Genetics 69% · Genome sequencing 75% · Sequencing 75%
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.34523 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:3105 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (69%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:genome-sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |