Data for: Reversible perturbation of the intestinal barrier–microbiota–immunity axis and nutritional homeostasis by a short-term gluten-free diet

The study used a Balb/c mouse model and set up an experimental protocol of three weeks of gluten free diet intervention and three weeks of recovery diet. Combining metagenomics, proteomics, histopathology, and in vitro cell models, the effects of short-term gluten free diet on the integrity of the intestinal barrier, gut microbiota composition, and mucosal immunity of healthy hosts were systematically evaluated.

The dataset includes: (1) basic physiological indicators such as mouse body weight, feed intake, and water intake; (2) Histological images of duodenal tissue stained with H&E and PAS, as well as measurement data of villus height/crypt depth; (3) TUNEL staining and transmission electron microscopy (TEM) images; (4) RT qPCR gene expression data (inflammatory factors, antioxidant enzymes, tight junction proteins); (5) Western blot protein expression data (tight junction protein, apoptotic pathway, TLR4/MyD88/NF - κ B pathway TGM2）； (6) Raw data and analysis results of metagenomic sequencing of cecal contents (species composition, alpha/beta diversity, differentially expressed species, LEfSe analysis); (7) Label free quantitative proteomics data and differential protein analysis results of duodenal tissue (GO/KEGG enrichment analysis); (8) Flow cytometry CD4+/CD8+T cell analysis data; (9) ELISA serological data (anti tTG IgA, anti DGP IgG); (10) Immunofluorescence staining images and quantitative data; (11) CCK-8, RT qPCR, and Western blot data of Caco-2+P31-43 in vitro cell model.