Transcriptome of the knockout mutant and control group of Synechococcus sp. PCC 7002 under 1.2 mol/L NaCl condition

After 24 h, the four groups of algal cells were collected by centrifugation at 12,000 rpm for 5 min. The supernatant was removed, and the samples were immediately flash‑frozen in liquid nitrogen and stored at −80°C until use. Total RNA was extracted, and RNA quality was assessed using a Nanodrop and Agilent 2100.

Integrity was checked by 1.5% agarose gel electrophoresis. Samples meeting the requirements were used for library construction.Sequencing and data quality control:Libraries were sequenced on an Illumina PE150 platform. Raw reads were quality‑filtered using fastp software.

The reference genome of Synechococcus sp. PCC 7002 was downloaded from the NCBI database (ncbi.nlm.nih.gov/datasets/genome/GCF_000019485.1). Clean reads after quality control were accurately aligned to the reference genome using HISAT2 software.

Read quantification was performed with the featureCounts function in the subread package. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) functional enrichment analyses of differentially expressed gene sets were carried out using the clusterProfile software (P < 0.05).